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Universal Staining Kit DAB, Biotin/Streptavidin System


Catalogue number: DA005-200

Units200 tests
Application IHC(P)
Background
Suitable for the detection of all primary antibodies of IgG type derived from mouse or rabbit. Application on human tissues tested, not applicable to mouse or rat tissues. For rat tissues we recommend Art. No. DA007 and Art. No. DA008 respectively, For other species please enquire. Except of primary antibodies BioPrime Universal Staining Kit DAB contains all components in a ready to use format, required for immunohistochemical staining reactions. By the use of a high affinity streptavidin/biotin system high sensitivity is achieved. The biotinylated secondary antibody is directed against mouse and rabbit IgG and is suitable for use in all human tissues and cells after the application of mouse and rabbit primary antibodies. The blocking solution enclosed is compatible with the secondary antibody and free of biotin.

Purification Method
Kit contents: 2 x 10 ml blocking solution, 2 x 10 ml biotinylated anti-mouse/rabbit-IgG, 2 x 10 ml streptavidin : HRPO, 4 ml DAB solution, 40 ml substrate buffer

Working Concentration
(RTU) neat

Incubation Time
30 min secondary antibody and streptavidin conjugate, 3-5 min substrate reaction

Product
Kit contents: 2 x 10 ml blocking solution, 2 x 10 ml biotinylated anti-mouse/rabbit-IgG, 2 x 10 ml streptavidin : HRPO, 4 ml DAB solution, 40 ml substrate buffer

Applications
IHC(P)

Cross Reactivity
Human

Storage
2-8°C

References


Caution
These staining kits are for research use only, they are not intended for clinical diagnostic use. *DAB, 3,3'-Diaminobenzidine, is potentially hazardous to health and may be used by trained staff only. Protect skin with laboratory gloves. After accidential contact with skin and mucous membranes wash with plenty of water and visit your physician. Disposal of waste according to national and local rules! R22; R36/37/38; S26; S36.CAS#: 91-95-2, EWG Nr. 202-110-6.

Universal Staining Kit DAB, Biotin/Streptavidin System

$806.00

SKU: DA005-200 Categories: ,