Background
CD71 is specific for the human transferrin receptor, MW 190 kdaltons (kDa), 90 kDa reduced. CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.

Source

Immunogen: Derived from the hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with blast cells from pokeweed mitogen-stimulated peripheral blood cells.

Product

Product Form: RPE

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Ammonium Sulfate Precipitation

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC. Antibodies should be stored at 4-8oC. Monoclonal antibodies should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Newman R, Schneider C, Sutherland R, Vodinelich L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982;1:397. 2. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press;1989:455-460. 3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1984;312:162-163. 4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980;152:1430. 5. Phillips JH, Le AM. Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J Exp Med. 1984;159:993-1008. 6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.

Protein Reference(s)

Database Name: UniProt

Accession Number: P02786

Safety Datasheet(s) for this product: