Background
Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders. The aim of this study was first to clarify the association between CD95 activation, hepatocyte apoptosis, and fulminant liver failure. Next, we investigated whether TNF-R1 and CD95 operate independently of each other in the induction of hepatocyte apoptosis.

Synonyms: hfas -PE, Anti human fas/Apo1 RPE CD95

Source

Immunogen: Full length CD95 protein

Product

Product Form: RPE

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Western Blotting

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1) The 55-kD tumor necrosis factor receptor and CD95 independently signal murine hepatocyte apoptosis and subsequent liver failure. Author : Leist M; Gantner F; K ̈unstle G; Bohlinger I; Tiegs G; Bluethmann H; Wendel A Source : Mol Med, 2(1):109-24 1996 Jan 2) Regulation of CD95 ligand expression: a key element in immune regulation? Author : Brunner T; Yoo NJ; Griffith TS; Ferguson TA; Green DR Source : Behring Inst Mitt, (97):161-74 1996 Oct 3)Expression of CD95 antigen and Bcl-2 protein in non-Hodgkin's lymphomas and Hodgkin's disease [published erratum appears in Am J Pathol 1996 Jul;149(1):346] Author : Nguyen PL; Harris NL; Ritz J; Robertson MJ Source : Am J Pathol, 148(3):847-53 1996 Mar 4) Relation of oxidative stress and glutathione synthesis to CD95(Fas/APO-1)-mediated apoptosis of adult T cell leukemia cells. Author : Kohno T; Yamada Y; Hata T; Mori H; Yamamura M; Tomonaga M; Urata Y; Goto S; Kondo T Source : J Immunol, 156(12):4722-8 1996 Jun 15 5) Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand. Author : M ̈uller KP; Mariani SM; Matiba B; Kyewski B; Krammer PH Source : Eur J Immunol, 25(10):2996-9 1995 Oct 6) Activation of the CD95 system increases with disease progression in human immunodeficiency virus type 1-infected children and adolescents. Author : B ̈ohler T; B ̈aumler C; Herr I; Groll A; Kurz M; Debatin KM Source : Pediatr Infect Dis J, 16(8):754-9 1997 Aug 7) Fc gammaRIII-mediated regulation of hematopoiesis in murine bone marrow cells by interleukin-3 and CD95 (Fas/Apo-1). Author : Yoshikawa H; Sakihama T; Nakajima Y; Tasaka K Source : Blood, 90(5):1911-9 1997 Sep 1 8) Human autoreactive and foreign antigen-specific T cells resist apoptosis induced by soluble recombinant CD95 ligand. Author : Zipp F; Martin R; Lichtenfels R; Roth W; Dichgans J; Krammer PH; Weller M Source : J Immunol, 159(5):2108-15 1997 Sep 1 9) The CD95 (APO-1/Fas) system mediates drug-induced apoptosis in neuroblastoma cells. Author : Fulda S; Sieverts H; Friesen C; Herr I; Debatin KM Research Center, Heidelberg. Source : Cancer Res, 57(17):3823-9 1997 Sep 1 10) Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells. Author : Karawajew L; Wuchter C; Ruppert V; Drexler H; Gruss HJ; D ̈orken B; Ludwig W. D. Source : Leukemia, 11(8):1245-52 1997 Aug

Protein Reference(s)

Database Name: UniProt

Accession Number: P25445 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: