Accelerating the Pace of Discovery

Kits

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labour intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.

A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analogue replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.

Apoptosis, also known as programmed cell death or ankoikis, leads to the elimination of cells without releasing harmful substances into the surrounding area. Apoptosis is the result of a cascade of molecular and biochemical events involving endogenous endonucleases that cleave DNA into the prototypical ‘ladder of DNA fragments’. In addition to producing classical DNA ladders, the apoptotic endonucleases generate free 3’-OH groups at the ends of these DNA fragments to allow for the detection of apoptotic cells using a molecular biology-based, end-labelling technique.

Kit Manuals

Our extensive range of kits can support your research whatever your area of interest.

Code Name Product Type Clone
10613002 Double-stranded RNA (dsRNA) ELISA kit (J2 based) ELISA Kit J2/K2
AE002 Peroxidase Substrate Kit AEC, 2 Components
10623002 Double-stranded RNA (dsRNA) ELISA kit (K1 based) ELISA Kit K1/K2
10623005 Double-stranded RNA (dsRNA) ELISA kit (K1 based) ELISA Kit K1/K2
X1545K BrdU Immunohistochemistry Kit Immunohistochemistry Kit
X1545K.1 BrdU Immunohistochemistry Kit Kits
X1623K3 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X1327K1 BrdU Cell Proliferation Assay Kit Assay Kit
X1327K2 BrdU Cell Proliferation Assay Kit Assay Kit
X1327K3 BrdU Cell Proliferation Assay Kit Assay Kit
X1623K1 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X1623K2 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X2044K1 DNA Fragmentation Detection Kit Immunohistochemistry Kit
X2044K2 DNA Fragmentation Detection Kit Immunohistochemistry Kit
IK 2000 EggsPress IgY Purification Kit (20 yolks) Kits
IK 4000 EggsPress IgY Purification Kit (40 yolks) Kits
IK 8000 EggsPress IgY Purification Kit (80 yolks) Kits
IK 500 EggsPress IgY Purification Kit (5 yolks) Kits
10613005 Double-stranded RNA (dsRNA) ELISA kit (J2 based) ELISA Kit J2/K2
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