Control antibodies are a vital part of assay design and ensure signal detected during antibody-based applications, can be attributed to specific antigen binding, rather than non-specific background staining.
Negative isotype controls function by determining the level of non-specific binding of the primary antibody to target. This is achieved by matching the control antibody host species, isotype, and if applicable fluorochrome, to that of the experimental specific antibody. A matched fluorochrome is required if the experimental primary antibody is directly conjugated. Negative control antibody is deliberately generated against a chemical or target not expressed by the cell or tissue of interest, so any binding will be non-specific in nature. This non-specific binding is most frequently caused by Fc receptors expressed on the surface of the target sample. These receptors bind the antibody’s Fc region independently of their Fab epitope binding domains.
Control antibodies are most commonly used in flow cytometry or immunohistochemistry to determine levels of background staining, but can also be utilized as blocking agents before addition of primary antibody.
Code | Name | Product Type | Clone |
---|---|---|---|
BAlb | Bovine Serum albumin | Proteins & Peptides | |
BGG | Bovine gamma globulin Fr.II | Proteins & Peptides | |
Bii-rGFP-100 | Purified recombinant Green Fluorescent Protein (wild type) | Proteins & Peptides | |
BSA | Bovine Serum albumin Fr.V | Proteins & Peptides | |
BSA/FITC | Bovine Serum albumin Fr.V, conjugated with FITC | ||
BSA/TRITC | Bovine Serum albumin Fr.V, conjugated with TRITC | ||
HSA | Human Serum albumin Fr.IV | Proteins & Peptides | |
HuAlb | Human Serum albumin | Proteins & Peptides | |
HuFab | Human Fab of normal IgG | Proteins & Peptides | |
P 016 | Human Bence Jones kappa | Proteins & Peptides | |
P 017 | Human Bence Jones lambda | Proteins & Peptides | |
P 020 | Human secretory IgA | Proteins & Peptides | |
MAlb | Mouse Serum albumin | Proteins & Peptides | |
MIgG | Mouse IgG | Proteins & Peptides | |
P 430 | Human IgA1 kappa, polymer | Proteins & Peptides | |
PO | Horseradish peroxidase | Proteins & Peptides | |
RAlb | Rabbit Serum Albumin | Proteins & Peptides | |
X1632B | Sphingosine Kinase 1 Blocking Peptide | Blocking Peptide | |
X2050B | Adiponectin Receptor 1 | Blocking Peptide | |
X1098 | Collagen Substrate, FITC Conjugate | Proteins & Peptides | N/A |
X1573B | Lysophosphatidic Acid Receptor 2 (EDG4) CT | Blocking Peptide | |
X1642B | Sphingosine Kinase 2 Blocking Peptide | Blocking Peptide | |
X1645B | Sphingosine 1-Phosphate Phosphatase 2 | Blocking Peptide | |
X1644B | Sphingosine 1-Phosphate Phosphatase 1 | Blocking Peptide | |
X1646B | GPR4 Blocking Peptide | Blocking Peptide | |
X1852B | FHL2 (SLIM3) | Blocking Peptide | |
X1854B | SIAH1, E3 Ubiquitin Ligase | Blocking Peptide | |
X1853B | Sulf2 | Blocking Peptide | |
X1855B | SPARC | Blocking Peptide | |
X1868b | Netrin 1 | Blocking Peptide | |
X1869B | Trag-3 | Blocking Peptide | |
X1875B | Sphingosine Kinase 1, Long Form, Rat | Blocking Peptide | |
X1876B | HDAC1, Human | Blocking Peptide | |
X1877B | Sphingosine Kinase 1 (Ser225), Mouse | Blocking Peptide | |
X2372B | Monocarboxylate Transport 2 | Blocking Peptide | |
X2375B | G-Protein Coupled Receptor 94 | Blocking Peptide | |
X2377B | SMO (Smoothened Homolog) | Blocking Peptide | |
X2378B | SET, Phosphatase 2A Inhibitor I2PP2A | Blocking Peptide | |
X2380B | Ceramide Transfer Protein (CERTL) | Blocking Peptide | |
X2382B | ELOVL4 | Blocking Peptide |