Catalogue

Background
CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.

Synonyms: CD8 FITC - CD56 PE

Source

Immunogen: CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Product

Product Form: Bi-Test (FITC/RPE) Reagent

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Evolutionary Conservation of Surface Molecules that Distinguish T Lymphocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. Ledbetter,J.A., Evans,R.L., Lipinski,M., Cunningham-Rundles,C., Good,R.A. and Herzenberg,L.A., J. Exp. Med. 153,310, 1981. 2. Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Engleman,E.G.,Benike,C.J.,and Evans,R.L., Clin. Res. 29, 365a 1981. 3. Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. Kotzin,B.L., Benike,C.J. and Engleman,E.G., J. Imm. 127,931,1981. 4. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Gallagher,P.F., Fazekas de St. Groth,B., Miller, J.F.A.P., Proc. Nat. Acad. Sci. 1989,86:10044. 5. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. 6. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. 7. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. Hercend, T., Griffin, J.D., Benussan, A., Schmidt, R.E., Edson, M.A., Brennen, A., Murray, C., Daley, J.F., Schlossman, S.F., and Ritz, J.. J. Clin. Invest. 1985 75:932. 8. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood Nk Cells and Cytotoxic T Lymphocytes. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H., J. Immunol. 1986 136,4480. 9. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J Ex Med 1986 164: 351-356 10. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J, J Immunol 1987 139:3306-3319 11. Hercend T., and Schmidt, R.E., Immunol. Today 9,291,1988.

Protein Reference(s)

Database Name: UniProt

Accession Number: P01732, P13591

Safety Datasheet(s) for this product: