Background
CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. Exalpha's CD56, clone C5.9 has been recognized as the superior CD56 antibody for flow cytometry applications because of its specific and intense staining.

Synonyms: CD56, NCAM1, Neural Cell Adhesion Molecule

Source
Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Immunogen: Extract from KG1a cell line.

Product
PerCp conjugated monoclonal antibody C5.9, rovided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Product provided titered for use in flow cytometry when used as indicated.

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P13591

Safety Datasheet(s) for this product: