Accelerating the Pace of Discovery

Secondary Antibodies

Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufactures and supplies polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
GAHU/IgG(Fc)/FITC Goat anti Human IgG (Fc specific), conjugated with FITC Secondary Antibodies Polyclonal
GAM/IgE(Fc) Goat anti Mouse IgE (Fc specific) Polyclonal
GAM/IgE(Fc)/7S Goat anti Mouse IgE (Fc specific) Polyclonal
GAM/IgE(Fc)/Bio Goat anti Mouse IgE (Fc specific), conjugated with Biotin Polyclonal
GAM/IgE(Fc)/PO Goat anti Mouse IgE (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAM/IgE(Fc)/TRITC Goat anti Mouse IgE (Fc specific), conjugated with TRITC Polyclonal
GAM/IgG(Fc) Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific) Polyclonal
GAM/IgG(Fc)/7S Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific) Polyclonal
GAM/IgG(Fc)/Bio Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with Biotin Polyclonal
GAM/IgG(Fc)/FITC Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC Polyclonal
GAM/IgG(Fc)/PO Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAM/IgG(Fc)/TRITC Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with TRITC Polyclonal
GAM/IgG(H+L) Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains) Polyclonal
GAM/IgG(H+L)/7S Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains) Polyclonal
GAM/IgG(H+L)/Bio Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains), conjugated with Biotin Polyclonal
GAM/IgG(H+L)/FITC Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains), conjugated with FITC Polyclonal
GAM/IgG(H+L)/PO Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GAM/IgG(H+L)/TRITC Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains), conjugated with TRITC Polyclonal
GAM/IgG1 Goat anti Mouse IgG1 (subclass specific) Polyclonal
GAM/IgG1/7S Goat anti Mouse IgG1 (subclass specific) Polyclonal
GAM/IgG1/Bio Goat anti Mouse IgG1 (subclass specific), conjugated with Biotin Polyclonal
GAM/IgG1/FITC Goat anti Mouse IgG1 (subclass specific), conjugated with FITC Polyclonal
GAM/IgG1/PO Goat anti Mouse IgG1 (subclass specific), conjugated with Horseradish peroxidase Polyclonal
GAM/IgD(Fc)/FITC Goat anti Mouse IgD (Fc specific), conjugated with FITC Polyclonal
GAM/IgG2/7S Goat anti Mouse IgG2 (subclass specific) Polyclonal
GAM/IgG2/FITC Goat anti Mouse IgG2 (subclass specific), conjugated with FITC Polyclonal
GAM/IgG2/PO Goat anti Mouse IgG2 (subclass specific), conjugated with Horseradish peroxidase Polyclonal
GAM/IgG2/TRITC Goat anti Mouse IgG2 (subclass specific), conjugated with TRITC Polyclonal
GAM/IgG2/Bio Goat anti Mouse IgG2 (subclass specific), conjugated with Biotin Polyclonal
GAM/IgG2a Goat anti Mouse IgG2a (subclass specific) Polyclonal
GAM/IgG2a/7S Goat anti Mouse IgG2a (subclass specific) Polyclonal
GAM/IgG2a/Bio Goat anti Mouse IgG2a (subclass specific), conjugated with Biotin Polyclonal
GAM/IgG2a/FITC Goat anti Mouse IgG2a (subclass specific), conjugated with FITC Polyclonal
GAM/IgG2a/PO Goat anti Mouse IgG2a (subclass specific), conjugated with Horseradish peroxidase Polyclonal
GAM/IgG2a/TRITC Goat anti Mouse IgG2a (subclass specific), conjugated with TRITC Polyclonal
GAM/IgG2ab Goat anti Mouse IgG2ab (subclass specific) Polyclonal
GAM/IgG2b Goat anti Mouse IgG2b (subclass specific) Polyclonal
GAM/IgG2b/7S Goat anti Mouse IgG2b (subclass specific) Polyclonal
GAM/IgG2b/Bio Goat anti Mouse IgG2b (subclass specific), conjugated with Biotin Polyclonal
GAM/IgG2b/FITC Goat anti Mouse IgG2b (subclass specific), conjugated with FITC Polyclonal
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