Accelerating the Pace of Discovery

Secondary Antibodies

Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufactures and supplies polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
GARa/IgG2c/TRITC Goat anti Rat IgG2c (subclass specific), conjugated with TRITC Polyclonal
GARa/IgM(Fc) Goat anti Rat IgM (Fc specific) Polyclonal
GARa/IgM(Fc)/7S Goat anti Rat IgM (Fc specific) Polyclonal
GARa/IgM(Fc)/Bio Goat anti Rat IgM (Fc specific), conjugated with Biotin Polyclonal
GARa/IgM(Fc)/FITC Goat anti Rat IgM (Fc specific), conjugated with FITC Polyclonal
GARa/IgM(Fc)/PO Goat anti Rat IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GARa/IgM(Fc)/TRITC Goat anti Rat IgM (Fc specific), conjugated with TRITC Polyclonal
GARa/L(SD+HD) Goat anti Rat Ig light chains kappa and lambda (free and bound) Polyclonal
GARa/mIg/7S Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse) Polyclonal
GARa/mIg/Bio Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse), conjugated with Biotin Polyclonal
GARa/mIg/FITC Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse), conjugated with FITC Polyclonal
GARa/mIg/PO Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse), conjugated with Horseradish peroxidase Polyclonal
GARa/mIg/TRITC Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse), conjugated with TRITC Polyclonal
GASw/IgA(Fc) Goat anti Swine IgA (Fc specific) Polyclonal
GASw/IgG(Fc) Goat anti Swine IgG (Fc specific) Polyclonal
GASw/IgG(H+L) Goat anti Swine IgG (heavy and light chains) Polyclonal
GASw/IgG(H+L)/PO Goat anti Swine IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GASw/IgM(Fc) Goat anti Swine IgM (Fc specific) Polyclonal
GASw/IgM(Fc)/Bio Goat anti Swine IgM (Fc specific), conjugated with Biotin Polyclonal
GASw/IgM(Fc)/PO Goat anti Swine IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GATu/IgG(H+L) Goat anti Turkey IgG (heavy and light chains) Polyclonal
GATu/IgG(H+L)/Bio Goat anti Turkey IgG (heavy and light chains), conjugated with Biotin Polyclonal
GATu/IgG(H+L)/FITC Goat anti Turkey IgG (heavy and light chains), conjugated with FITC Polyclonal
GATu/IgG(H+L)/PO Goat anti Turkey IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
IG515 Ig Lambda Chain, human HP-6054
M/PAP Mouse peroxidase-anti-peroxidase Polyclonal
MAHu/BJK Mouse anti Human Bence Jones kappa (surface and hidden determinants) NI 285, NI 250
MAHu/BJK/Bio Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with Biotin Monoclonal Antibody NI 285, NI 250
MAHu/BJK/FITC Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with FITC NI 285, NI 250
MAHu/BJK/MAb Mouse anti Human Bence Jones kappa (surface and hidden determinants) NI 285, NI 250
MAHu/BJK/PO Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with Horseradish peroxidase NI 285, NI 250
MAHu/BJK/TRITC Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with TRITC NI 285, NI 250
MAHu/BJL Mouse anti Human Bence Jones lambda (surface and hidden determinants) NI 412, NI 268
MAHu/BJL/Bio Mouse anti Human Bence Jones lambda (surface and hidden determinants), conjugated with Biotin Monoclonal Antibody NI 412, NI 268
MAHu/BJL/MAb Mouse anti Human Bence Jones lambda (surface and hidden determinants) NI 412, NI 268
MAHu/BJL/PO Mouse anti Human Bence Jones lambda (surface and hidden determinants), conjugated with Horseradish peroxidase NI 412, NI 268
MAHu/Ig Set Mouse anti Human IgG IgA IgM IgD IgE (class specific) Mixed
MAHu/IgA Set Mouse anti Human IgAc IgA1 IgA2 secretory component Mixed
MAHu/IgA1 Mouse anti Human IgA1 (subclass specific) NI 69-11 (A89-036)
MAHu/IgA1/Bio Mouse anti Human IgA1 (subclass specific), conjugated with Biotin NI 69-11 (A89-036)
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