CD36, human

Catalogue number: CD36-1

Clone	NF189
Isotype	IgG1
Units	50 µg
Host	Mouse
Application	Flow Cytometry

Background
The antibody clone NF189 is well suited for the detection of CD36-positive cells and the differentiation from other cell populationes (e.g. lymphocytes and granulocytes). CD36 is a 88 kDa membrane glycoprotein, which is mainly exprimed by thrombocytes, monocytes and macrophages. It is also present in endothelia of small blood vessels of the liver, brain, thyroid and kidney as well as ductal epithelia of the breast, adipocytes (rat) and haematopoietic precursor cells. CD36 functions as a receptor for TSP (thrombospondin), endothelial receptor for Malaria infected erythrocytes (Sickle Cells) and in monocytes / macrophages as a receptor for oxidised LDL. Human CD36 molecule (GPIV, GPIIIb).

Source

Immunogen: Human platelet membrane fraction

Product
Protein G affinity purified antibody isolated from ascites buffer contains 2% Block Ace and 0,1% Proclin as preservatives

Purification Method: Protein G affinity purified antibody isolated from ascites buffer contains 2% Block Ace and 0,1% Proclin as preservatives

Concentration: 250 µg/ml

Secondary Reagents: We recommend the use of anti-mouse-IgG:FITC (e.g. art. no. FI-2000) or anti-mouse-IgG:RPE (e.g. art. no. EI-2000).

Specificity

Species Reactivity: Human

Applications
FACS

Incubation Time: 60 min at RT or 18 hr at 2-8°C

Working Concentration: (liquid conc.) 0.5-20µg/ml (FACS)

Positive Control: Human monocytes

Storage
-20

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References