CA 15-3 (CD227, MUC-1)Catalogue number: CA153-1
In tumours strong staining is observed in breast carcinoma, carcinoma of the gastro intestinal tract, lung, kidneys, bladder and the ovary. Additionally mesothelioma, synovial sarcoma and malign fibrous histiocytoma are CA 15-3-positive. In mamma carcinoma usually a cytoplasmic distribution of the antigen is found, while in benign tumours the antigen is typically located in the apical part of the cells. In normal cells the antibody to CA 15-3 labels a mucinous glycoprotein (MUC-1) of glandular epithelial cells of the breast, colon, kidney, liver, lung, pancreas and salivary glands. Additionally fibroblasts of stroma and smooth muscle cells of vessels may be stained Human mamma carcinoma CA 15-3 (MUC-1, CD227). No cross-reactivity to other tumor-associated antigens like AFP, CEA, PAP, PSA, CA19-9 and CA125..
Immunogen: Purified urinary MUC-1
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Concentration: 100 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
IHC(C, P), ELISA, WB
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:20 - 1:100
Pre-Treatment: In paraffin section no pre-treatment is required, however heat induced antigen retrieval may improve the staining. Each laboratory should select it's own optimum staining and pre-treatment conditions.
Positive Control: Mamma carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Price, M.R. et al. (1990). Immunological and structural features of the protein core of human polymorphic epithelial mucin. Molec. Immunol. 27: 795-802. 2. Price, M. R. et al. (1990). C595 - a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas. Br. J. Cancer. 61: 681. 3. Gatalica Z., and Miettienen M. (1994) Distribution of carcinoma antigens CA 19-9 and CA 15-3: an immunohistochemical study of 400 tumors. Appl. Immunohistochem. 2(3); 205ff. 4. Denton, G. et al. (1995). Primary sequence determination and molecular modelling of the variable region of an anti MUC1 mucin monoclonal antibody. Eur. J. Cancer 31A: 214-221. 5. Denton, G. et al. (1997). Production and characterization of a recombinant anti Muc 1 scFv reactive with human carcinomas. Br. J. Cancer. 76 (5): 614-21.
Database Name: UniProt
Accession number: P15941 MUC1_Human
Species Accession: Human
CA 15-3 (CD227, MUC-1)