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C-erbB-2 Onkoprotein, HER-2/neu


Catalogue number: CE110-0.5

CloneZR5
IsotypeIgG
Units0.5 ml
HostRabbit
Application IHC(C
P)
Background
The antibody may be used for the detection of over-expression of C-erbB-2 oncoprotein in mamma carcinoma and other adenocarcinoma. C-erbB-2 proto-oncogene is localized on human chromosome 17 encoding for a 185-190 kDa membrane protein, showing structural similarity to the EGF-receptor. Over-expression of c-erbB-2 oncoprotein is correlated with bad prognosis in mamma carcinoma. Also other carcinoma like adenocarcinoma of the stomach exhibit increased levels of C-erb B-2 gene product. Synonyms for c-erbB-2 oncoprotein are ERBB2 oncoprotein, neu-oncoprotein, and HER-2/neu oncoprotein. C-erbB-2 oncoprotein.

Positive Control
Mamma carcinoma

Pre-Treatment
Improvement of staining with Unmasking Fluid E (Art. No. DE006) or Unmasking Fluid T (Art. No. DE005)

Immunogen
Recombinant protein according to the extracellular domain of the c-erbB2 molecule

Purification Method
Cell culture medium with BSA and Sodium Azide as preservative

Working Concentration
(liquid conc.) 1:200

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Cell culture medium with BSA and Sodium Azide as preservative

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Van de Vijver M. J., et al. (1988) Immunohistochemical detection of the neu-protein in tissue sections of human breast tumours with amplified neu-DNA. Oncogene 2; 175-178. 2. Flack V.G. et al., et al. (1989) C-erb B-2 oncogene product staining in gastric adenocarcinoma. An immunohistochemical study. J. Pathol. 159; 107-111. 3. May E., Mouriesse H., May-Levin F., Quian J.F., May P., and Delarue J.C. (1990) Human breast cancer: Identification of populations with a high risk of early relapse in relation to both oestrogen receptor status and c-erbB-2 overexpression. Br. J. Cancer 62; 430-435. 4. Soomro S., Shousha S., Taylor P., Shephard H.M., Feldmann M. (1991) C-erbB-2 expression in different histological types of invasive breast carcinoma. J. Clin. Pathol. 44; 211-214. 5. Xia W ; Lau YK ; Zhang HZ ; Liu AR ; Li L ; Kiyokawa N ; Clayman GL ; Katz RL ; Hung MC TI (1997)- Strong correlation between c-erbB-2 overexpression and overall survival of patients with oral squamous cell carcinoma. - Clin Cancer Res 3(1):3-9.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

C-erbB-2 Onkoprotein, HER-2/neu

$854.00

SKU: CE110-0.5 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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