C-erbB-2 Onkoprotein, HER-2/neuCatalogue number: CE110-0.5
The antibody may be used for the detection of over-expression of C-erbB-2 oncoprotein in mamma carcinoma and other adenocarcinoma. C-erbB-2 proto-oncogene is localized on human chromosome 17 encoding for a 185-190 kDa membrane protein, showing structural similarity to the EGF-receptor. Over-expression of c-erbB-2 oncoprotein is correlated with bad prognosis in mamma carcinoma. Also other carcinoma like adenocarcinoma of the stomach exhibit increased levels of C-erb B-2 gene product. Synonyms for c-erbB-2 oncoprotein are ERBB2 oncoprotein, neu-oncoprotein, and HER-2/neu oncoprotein. C-erbB-2 oncoprotein.
Immunogen: Recombinant protein according to the extracellular domain of the c-erbB2 molecule
Cell culture medium with BSA and Sodium Azide as preservative
Purification Method: Cell culture medium with BSA and Sodium Azide as preservative
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:200
Pre-Treatment: Improvement of staining with Unmasking Fluid E (Art. No. DE006) or Unmasking Fluid T (Art. No. DE005)
Positive Control: Mamma carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Van de Vijver M. J., et al. (1988) Immunohistochemical detection of the neu-protein in tissue sections of human breast tumours with amplified neu-DNA. Oncogene 2; 175-178. 2. Flack V.G. et al., et al. (1989) C-erb B-2 oncogene product staining in gastric adenocarcinoma. An immunohistochemical study. J. Pathol. 159; 107-111. 3. May E., Mouriesse H., May-Levin F., Quian J.F., May P., and Delarue J.C. (1990) Human breast cancer: Identification of populations with a high risk of early relapse in relation to both oestrogen receptor status and c-erbB-2 overexpression. Br. J. Cancer 62; 430-435. 4. Soomro S., Shousha S., Taylor P., Shephard H.M., Feldmann M. (1991) C-erbB-2 expression in different histological types of invasive breast carcinoma. J. Clin. Pathol. 44; 211-214. 5. Xia W ; Lau YK ; Zhang HZ ; Liu AR ; Li L ; Kiyokawa N ; Clayman GL ; Katz RL ; Hung MC TI (1997)- Strong correlation between c-erbB-2 overexpression and overall survival of patients with oral squamous cell carcinoma. - Clin Cancer Res 3(1):3-9.
C-erbB-2 Onkoprotein, HER-2/neu