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Mouse anti Human B7.1 (extra-cellular domain)


Catalogue number: 0861

Synonyms
CD86, mouse Anti Human B7.2 unconjugated

CloneB72-H2
IsotypeIgG2a
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Human
Application Flow Cytometry
Immunohistochemistry
Product Form
Unconjugated

Background
Cell Distribution: CD86 antibody is rapidly induced on the surface of and is expressed at high levels on dendritic cells. It is also found on malignant Hodgkin and Reed-Sternberg (H-RS) cells in HodgkinÕs disease. Function: CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient costimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation.

Positive Control
Specificity: Recognizes the extra-cellular domain of human CD86 (also called B7-2). Does not bind the extracellular domain of CD80.

Immunogen
Recombinant expressed extra-cellular domain of human CD86.

Purification Method
Protein A/G Chromatography

Purity
> 95 % by SDS PAGE

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting, Flow cytometry, Immunohistochemistry.

Applications
Antibody can be used for flow cytometry and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Chan, E.P.H., et al. 'Effects of titanium(IV) ions on human monocyte-derived dendritic cells.' Metallomics 2009, 1, 166-174.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Mouse anti Human B7.1 (extra-cellular domain)

$294.00

SKU: 0861 Categories: ,