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DNA Fragmentation Detection Kit

$423.00

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SKU: X2044K2 Categories : Kits, Apoptosis kits, Immunohistochemistry Kit
Figure 2. DNA Fragmentation Detection Kit (X2044K) using paraffin fixed human tonsil tissue, 10 μm sections (1000X). [A] Section processed and counter-stained with methyl green according to the DNA Fragmentation Detection Kit manual. [B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centers of tonsil tissue. [C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3’-OH groups on cellular DNA, these free 3’-OH groups are then labeled with biotin-nucleotide by the TdT in the DNA Fragmentation Detection Kit (X2044K). [D] Negative control, where the TdT enzyme step was eliminated, thereby generating a negative slide.

Figure 2. DNA Fragmentation Detection Kit (X2044K) using paraffin fixed human tonsil tissue, 10 μm sections (1000X). [A] Section processed and counter-stained with methyl green according to the DNA Fragmentation Detection Kit manual. [B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centers of tonsil tissue. [C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3’-OH groups on cellular DNA, these free 3’-OH groups are then labeled with biotin-nucleotide by the TdT in the DNA Fragmentation Detection Kit (X2044K). [D] Negative control, where the TdT enzyme step was eliminated, thereby generating a negative slide.