Background
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP1-3. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas1,3.

Synonyms: Anti-Mouse Survivin (C-terminal); Anti-Mouse TIAP (C-terminal); Anti-Mouse API4 (C-terminal)

Source

Immunogen: Synthetic peptide corresponding to amino acids 128 to 140 of mouse survivin protein.

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Detects survivin protein by Western blot at 0.5 to 1 µg/ml. Detects 17 kDa band in mouse spleen tissue lysate. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Mouse spleen tissue lysate

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Ambrosini, G., et al. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat. Med. 1997, 3, 917-921 2. Li, F., et al. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature 1998, 396, 580-584 3. Kobayashi, K., et al. Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation. Proc. Natl. Acad. Sci. USA 1999, 96, 1457-1462

Protein Reference(s)

Database Name: SwissProt

Accession Number: O70201

Species Accession: Mouse

Safety Datasheet(s) for this product: