Rabbit anti Human Gi alpha 1/2Catalog number: X1595P
GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of -subunits ( i1, i2 and i3)
Rabbits were immunized with a peptide corresponding to amino acids 345-354 of Gi 1 and 346-355 of Gi 2.
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Concentration: See vial for concentration
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: IMR-5 cells or brain
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Li X, et al. Pertussis toxin-sensitive G protein alpha-subunits: production of monoclonal antibodies and detection of differential increases on differentiation of PC12 and LA-N-5 cells. J. Neurochem. 1995, 64, 1107-1117.
Database Name: SwissProt
Accession Number: P04899
Species Accession: Human
Safety Datasheet(s) for this product:
Rabbit anti Human Gi alpha 1/2
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" Western blot analysis using Giα1/2 antibody on 10 ng of purified Giα1 protein."