Catalogue

Background
Voltage-dependent calcium channels (VDCCs) are large (>400 kDa) heteromers which contain, minimally, three core subunits 1, 2/ , in a 1:1:1 stoichiometry1. Expression of VDCC gene products in Xenopus oocytes, or transfected cells shows that the 1 subunits contain the ion channel pore while the auxiliary 2/ and subunits confer optimal cell surface expression and channel kinetics1. Until recently, the only exception to the above paradigm was the skeletal muscle VDCC, which, in addition to the 1, 2/ , core motif, also has an additional tightly associated integral membrane glycoprotein subunit termed 1. Upon co-expression with the 1.1, 2/ 1, 1a subunits of the skeletal muscle VDCC, subunits alter the peak currents, and the kinetics of channel activation and inactivation with the overall effect being a normalisation of currents to those resembling the endogenous channel2. Together, these results suggest that subunits modulate skeletal muscle VDCCs by stabilising their conformation. The 4 subunit is specifically localized in the brain, with the 2 and 3 subunits. It shares >60% sequence homology with the 2 and 3 subunits and ~25% sequence homology with the 1 and 5 subunits.

Synonyms: Neuronal voltage-gated calcium channel gamma-4 subunit; Transmembrane AMPAR regulatory protein gamma-4; Voltage-dependent calcium channel gamma-4 subunit

Source

Immunogen: Synthetic peptide derived from the rat calcium channel gamma4 subunit conjugated to KLH

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
This antibody can be used for Western blotting at 5-10 µg/ml. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat brain lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Hofmann F, et al. Rev Physiol Biochem Pharmacol 1999, 139:33-87 2. Singer D, et al. Science 1991, 253:1553-7 3. Letts VA, et al. Nat Genet 1998, 19:340-7 4. Kang, M.G., et al. J. Biol. Chem. 2001, 276: 32917-24 5. Sharp, A.H., et al. Neuroscience 2001, 105:599-617 6. Steinlein OK, Noebels JL Curr Opin Genet Dev 2000,10:286-91 7. Chen, L., et al. 'Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms.' Nature 2000, 408: 936-943.

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q9UBN1

Species Accession: Human

Safety Datasheet(s) for this product: