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Mouse anti Human CD56

$329.00

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SKU: 0561 Categories : Primary antibodies, Primary antibodies, Monoclonal Antibody
IHC: Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 ug/ml using peroxidase-conjugate and DAB chromogen. Cell membrane staining of tumor cells is clearly evident. High-temperature heating was used as the retrieval protocol, pressure cooker with citrate buffer, pH 6.5, for 10 min. Exalpha’s anti-CD56,clone C5.9, was added at 2 ug/ml for 1 hour at room temperature followed by anti-Mouse HRP for 30 minutes at room temperature. Staining was visualized using DAB and methyl green counter stain. Flow Cytometry: Flow cytometry data using Exalpha’s Mouse anti Human CD56 antibody 0561 at 8 µg/ml on human lymphocytes, with Rat anti Mouse PE IgG2a & 2b as a second step. The negative control was generated without the CD56 antibody and without the Rat anti Mouse PE IgG2a & 2b. Western Blot: Western blot analysis using Exalpha’s Mouse anti Human CD56 antibody 0561 at 10 µg/ml (left lane) and 1 µg/ml (right lane) showing a strong reaction on recombinant human NCAM-1/CD56 protein.

IHC: Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 ug/ml using peroxidase-conjugate and DAB chromogen. Cell membrane staining of tumor cells is clearly evident. High-temperature heating was used as the retrieval protocol, pressure cooker with citrate buffer, pH 6.5, for 10 min. Exalpha’s anti-CD56,clone C5.9, was added at 2 ug/ml for 1 hour at room temperature followed by anti-Mouse HRP for 30 minutes at room temperature. Staining was visualized using DAB and methyl green counter stain. Flow Cytometry: Flow cytometry data using Exalpha’s Mouse anti Human CD56 antibody 0561 at 8 µg/ml on human lymphocytes, with Rat anti Mouse PE IgG2a & 2b as a second step. The negative control was generated without the CD56 antibody and without the Rat anti Mouse PE IgG2a & 2b. Western Blot: Western blot analysis using Exalpha’s Mouse anti Human CD56 antibody 0561 at 10 µg/ml (left lane) and 1 µg/ml (right lane) showing a strong reaction on recombinant human NCAM-1/CD56 protein.