Background
CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.

Synonyms: CD11a FITC

Product

Product Form: FITC

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Protein kinase C-mediated regulation of the expression of CD14 and CD11/CD18 in U937 cells. Pedrinaci S; Ruiz-Cabello F; Gomez O; Collado A; Garr Int J Cancer 1990 Feb 1;45(2):294-8 2. Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils. Hartnell A; Moqbel R; Walsh GM; Bradley B; Kay AB Immunology 1990 Fe;69(2):264-70 3. Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Arnaout MA Immunol Rev 1990 Ap;114 p145-80 4. Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells. Scand J Immunol 1990 Au;32(2):137-47 5. Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. van Dijken PJ; Ghayur T; Mauch P; Down J; Burakoff SJ; Ferrara JL Transplantation 1990 Ma;49(5):882-6 6. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Patarroyo M; Prieto J; Rincon J; Timonen T; Lundberg C; Lindbom L; Asjo B; Gahmberg CG Immunol Rev 1990 Ap;114 p67-108 7. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins Anderson DC and Springer TA, Ann Rev Med 1987;38:175

Protein Reference(s)

Database Name: UniProt

Accession Number: P20701

Safety Datasheet(s) for this product: