Catalogue

Background
Antibody FLG01 is well suited for the detection of natural Filaggrin in immunohistochemistry. It is important to note, that some mutants may be undetectable due to sequence shifting. Filaggrins are a class of proteins, which are associated with intermediary filaments (keratins) in the differentiated epidermis of mammals. In a screening procedure for the reasons of innate pre-disposition of different skin diseases, rheumatoid arthritis, and asthma mutations were detected in gene (1q21). This gene encodes for Filaggrins. S.c. Loss-of-Function-Variants of the gene are associated with a severe form of Ichthyosis vulgaris (Fish scale disease), atopic dermatitis, eczema and asthma. In psoriasis the immunohistochemical staining pattern is changed, but the relation to the common European mutations of 1q21 has not been secured. Human Filaggrin 38 kDa and Pro-Filaggrin (app. 400 kDa).

Source

Immunogen: Recombinant human Filaggrin

Product
Protein G purified antibody in PBS pH 7.3 containing BSA and NaN3 (0.09%)

Purification Method: Protein G purified antibody in PBS pH 7.3 containing BSA and NaN3 (0.09%)

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Concentration: 50 µg/ml

Specificity

Species Reactivity: Human

Applications
IHC(P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 2-4 µg/ml

Pre-Treatment: For formaldehyde-fixed material we recommend antigen unmasking with citrate buffer (Unmasking Fluid C, art. no. DE000) at 90-100°C

Positive Control: Human skin, placenta or lens capsule

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. L J McKinley-Grant, W W Idler, I A Bernstein, D A Parry, L Cannizzaro, C M Croce, K Huebner, S R Lessin, and P M Steinert. Characterization of a cDNA clone encoding human filaggrin and localization of the gene to chromosome region 1q21.Proc Natl Acad Sci U S A. 1989 July; 86(13): 4848–4852. 2. Dutoit V, Fauchais AL, Hachulla E, Flipo RM, Prin L. Evaluation of anti-citrullinated filaggrin antibodies as hallmarks for the diagnosis of rheumatic diseases. Ann Rheum Dis. 2004 Apr;63(4):415-9. 3. Kurokawa I, Mizutani H, Kusumoto K, Nishijima S, Tsujita-Kyutoku M, Shikata N, Tsubura A. Cytokeratin, filaggrin, and p63 expression in reepithelialization during human cutaneous wound healing. Wound Repair Regen. 2006 Jan-Feb;14(1):38-45. 4. Palmer CN, Irvine AD, Terron-Kwiatkowski A, Zhao Y, Liao H,et al. (2006) Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet. 2006 Apr;38(4):441-6. 5. Holloway JW, Koppelman GH. Identifying novel genes contributing to asthma pathogenesis. Curr Opin Allergy Clin Immunol. 2007 Feb;7(1):69-74.

Protein Reference(s)

Database Name: UniProt

Accession Number: P20930 (FILA_HUMAN)

Species Accession: Human

Safety Datasheet(s) for this product: