Background
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulphated glycoprotein of approximately 150 kD.

Synonyms: Nidogen-1; Nid1

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from Fisher rat immunized with partially purified preparation of laminin from the EHS mouse tumor and mouse X63 Ag 8.653 myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody is suitable for immunoblotting (1:100-1:1000), immunocytochemistry and immunohistochemistry on frozen (and most likely also formalin fixed and paraffin embedded) tissues (1:25-1:200). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting, IHC

Positive Control: Mouse liver, lung, thymus, skin

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Ljubimov, A. V., et al. (1986). Basement membrane components produced by a mouse ascites teratocarcinoma TB24, Exp Cell Res 165, 530-40. 2. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-321. 3. Y. Horiguchi, J. -D. Fine, A. V. Ljubimov, H. Yamasaki, J. R. Couchman: Entactin: ultrastructural localization of an ubiquitous basement membrane glycoprotein in mouse skin. Archives of Dermatological Research, 1989, Volume 281, Issue 6, pp 427–432.

Safety Datasheet(s) for this product:
EA_Sodium Azide