Rat anti Mouse E-Selectin (CD62E)Catalog number: X1780M
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NF B dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations
Synonyms: CD62 antigen-line family member E; Endothelial leukocyte adhesion molecule 1
Immunogen: Hybridoma produced by the fusion of splenocytes from Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface and mouse Sp2/0 myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on frozen sections and flow cytometry (1:200 dilution). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: This antibody recognizes a conformational epitope in the CR1 region in murine E-selectin. Also partially inhibits leukocyte adhesion to murine activated endothelial cells.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Hallmann, R., et al. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. 2. Hammel, M., et al. (2001). Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74.
Database Name: UniProt
Accession Number: Q00690
Species Accession: Human
Safety Datasheet(s) for this product:
Rat anti Mouse E-Selectin (CD62E)
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