Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

CD56

  • Product Code: 0561
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $644

Cat #

0561		 Quantity:      

Data Sheet

Product Name

CD56

Synonyms

CD56, NCAM, Neural Cell Adhesion Molecule

Host/Source

Mouse

Clone

C5.9

Isotype

IgG2b

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

IHC (Paraffin), Flow Cytometry

Purification

Protein A/G Chromatography

Size

250 µg

Price (USD)

$644

Background

CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. Exalpha’s CD56, clone C5.9 has been recognized as the superior CD56 antibody for Flow Cytometry applications because of it’s specific and intense staining. In fact, our CD56 is bright enough to be used in FITC! Now this antibody is proven to work in formalin fixed paraffin embedded section. Available concentrated, pre-diluted in dropper bottles, or conjugated to several flurochromes for manual use or automated applications.

Immunogen

CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Formulation

Filtered includes 0.08% azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 ug/ml using peroxidase-conjugate and DAP chromogen. Cell membrane staining of tumor cells is clearly evident. High-temperature heating was used as the retrieval protocol, pressure cooker with citrate buffer, pH 6.5, for 10 min. Exalpha’s anti-CD56,clone C5.9, was added at 2 ug/ml for 1 hour at room temperature followed by anti-Mouse HRP for 30 minutes at room temperature. Staining was visualized using DAP and methyl green counter stain.

Database Links:

SwissProtP13591Human
SwissProtP13591Human
SwissProtP13591Human

References

1. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. Hercend, T., Griffin, J.D., Benussan, A., Schmidt, R.E., Edson, M.A., Brennen, A., Murray, C., Daley, J.F., Schlossman, S.F., and Ritz, J.. J. Clin. Invest. 1985 75:932.

2. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood Nk Cells and Cytotoxic T Lymphocytes. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H., J. Immunol. 1986 136,4480.

3. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J Ex Med 1986 164: 351-356

4. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J, J Immunol 1987 139:3306-3319

5. Hercend T., and Schmidt, R.E., Immunol. Today 9,291,1988.

6. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, The Jurnal of ClinicalEndocrinology & Metabolism, 2003, Vol 88(8) Pages 3921-3930,


Product Specific References


This product has been used in:

1. Horikawa, M., et al, 'Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity' Journal of Investigative Dermatology 2005, 125, , 731-737
2. Ishimoto, H., et al, 'Midkine, a heparin-binding growth factor, selectively stimulates proliferation of definitive zone cells of the human fetal adrenal gland' Journal of Endocrinol. Metab 2006, 91, , 4050-4056
3. Muench, M., et al, 'Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland' Journal of Clinical Endocrinol. Metab 2003, 88, , 3921-3930
4. Miles, L.A., et al, 'Cell-Surface Actin Binds Plasminogen and Modulates Neurotransmitter Release from Catecholaminergic Cells' Journal of Neuroscience 2006, 26, , 13017-13024