Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

CD56

  • Product Code: 0561
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $644

Cat #

0561		 Quantity:      

Data Sheet

Product Name

CD56

Synonyms

CD56, NCAM, Neural Cell Adhesion Molecule

Host/Source

Mouse

Clone

C5.9

Isotype

IgG2b

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

IHC (Paraffin), Flow Cytometry

Purification

Protein A/G Chromatography

Size

250 µg

Price (USD)

$644

Background

CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. Exalpha?s CD56, clone C5.9 has been recognized as the superior CD56 antibody for Flow Cytometry applications because of it?s specific and intense staining. In fact, our CD56 is bright enough to be used in FITC! Now this antibody is proven to work in formalin fixed paraffin embedded section. Available concentrated, pre-diluted in dropper bottles, or conjugated to several flurochromes for manual use or automated applications.

Immunogen

CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Formulation

Filtered includes 0.08% azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 ug/ml using peroxidase-conjugate and DAP chromogen. Cell membrane staining of tumor cells is clearly evident. High-temperature heating was used as the retrieval protocol, pressure cooker with citrate buffer, pH 6.5, for 10 min. Exalpha?s anti-CD56,clone C5.9, was added at 2 ug/ml for 1 hour at room temperature followed by anti-Mouse HRP for 30 minutes at room temperature. Staining was visualized using DAP and methyl green counter stain.

Database Links:

SwissProtP13591Human
SwissProtP13591Human
SwissProtP13591Human

References

1. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. Hercend, T., Griffin, J.D., Benussan, A., Schmidt, R.E., Edson, M.A., Brennen, A., Murray, C., Daley, J.F., Schlossman, S.F., and Ritz, J.. J. Clin. Invest. 1985 75:932.

2. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood Nk Cells and Cytotoxic T Lymphocytes. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H., J. Immunol. 1986 136,4480.

3. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J Ex Med 1986 164: 351-356

4. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J, J Immunol 1987 139:3306-3319

5. Hercend T., and Schmidt, R.E., Immunol. Today 9,291,1988.

6. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, The Jurnal of ClinicalEndocrinology & Metabolism, 2003, Vol 88(8) Pages 3921-3930,


Product Specific References


This product has been used in:

1. Horikawa, M., et al, 'Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity' Journal of Investigative Dermatology 2005, 125, , 731-737
2. Ishimoto, H., et al, 'Midkine, a heparin-binding growth factor, selectively stimulates proliferation of definitive zone cells of the human fetal adrenal gland' Journal of Endocrinol. Metab 2006, 91, , 4050-4056
3. Muench, M., et al, 'Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland' Journal of Clinical Endocrinol. Metab 2003, 88, , 3921-3930
4. Miles, L.A., et al, 'Cell-Surface Actin Binds Plasminogen and Modulates Neurotransmitter Release from Catecholaminergic Cells' Journal of Neuroscience 2006, 26, , 13017-13024