Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

CD4 PE

  • Product Code: 0044
  • Size: 100 Tests
  • Price (USD): $191

Cat #

0044		 Quantity:      

Data Sheet

Product Name

CD4 PE

Synonyms

T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Host/Source

Mouse

Clone

7E14

Isotype

IgG1

Product Type

Single-Color Reagent

Reactivity

Human

Applications

Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 Tests

Price (USD)

$191

Background

Identification of human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.

Immunogen

Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.

Formulation

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Product Image

Image Legend

Peripheral blood lymphocytes stained with Exalpha’s CD4 PE Conjugated Antibody (Cat. No. 0044).

Database Links:

SwissProtP01730Human

References

1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans,R.L., Wall,D.W., Platsoucas,C.D., Siegal,F.P., Fikrig,S.M., Testa,C.M, and Good,R.A. Proc. Nat. Acad. Sci. 78,544,1981.

2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement,L.T., Vink,P.E., Bradley,G.E. J. Immunology 145(1):102-8,1990 .

3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo,G., Miller,C.L., Kodys,K., J. Leukoc. Biol. 47(2): 111-20,1990.

4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond,D.C., Finberg,R., Chaudhuri,S., Sleckman,B.P., Burakoff,S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990.

5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins,B., J. Immunol. 146(5):1387-93,1991.

6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso,P., De Maria,A., Malnati,M., Lori,F., DeRocco,S.E., Baseler,M., Gallo,R.C., Nat. 349(6309):533-5,1991.

7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983.

8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987.

Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Product Specific References:

1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.


Product Specific References


This product has been used in:

1. O'Neill, E., et al, 'Dynamic Evolution of the Human Immunodeficiency Virus Type 1 Pathogenic Factor, Nef' Journal of Virology 2006, 80, 3, 1311-1320
2. Foster, J.L., et al, 'Genetic and Functional Diversity of Human Immunodeficiency Virus Type 1 Subtype B Nef Primary Isolates' Journal of Virology 2001, 75, , 1672-1680
3. Goldstein, S., et al, 'Plateau Levels of Viremia Correlate with the Degree of CD4+-T-Cell Loss in Simian Immunodeficiency Virus SIVagm-Infected Pigtailed Macaques: Variable Pathogenicity of Natural SIVagm Isolates' Journal of Virology 2005, 79, , 5153-5162
4. Chakrabarti, L.A., et al, 'A Truncated Form of Nef Selected during Pathogenic Reversion of Simian Immunodeficiency Virus SIVmac239{Delta}nef Increases Viral Replication' Journal of Virology 2003, 77, , 1234-1256
5. Chakrabarti, L.A., et al, 'Properties of the Surface Envelope Glycoprotein Associated with Virulence of Simian-Human Immunodeficiency Virus SHIVSF33A Molecular Clones' Journal of Virology 2002, 76, , 1588-1599
6. Luo, T., et al., 'Human Immunodeficiency Virus Type 1 Nef-Induced CD4 Cell Surface Downregulation Is Inhibited by Ikarugamycin' Journal of Virology 2001, 75, , 2488-2492
7. Anderson, S.J., et al, 'The cytoplasmic domain of CD4 is sufficient for its down-regulation from the cell surface by human immunodeficiency virus type 1 Nef' Journal of Virology 1994, 68, , 3092-3101
8. Kuo, L.S., et al. ‘Overlapping effector interfaces define the multiple functions of the HIV-1 Nef polyproline helix.’ Retrovirology, 9, 47 (2012)