Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

BrdU (Bromodeoxyuridine), FL549

  • Product Code: X2840K
  • Size: 50 Slides
  • Availability: In Stock In Stock
  • Price (USD): $376

Cat #

X2840K		 Quantity:      

Data Sheet

Product Name

BrdU (Bromodeoxyuridine), FL549

Synonyms

BrdU (Bromodeoxyuridine) Immunofluorescence Kit

Product Type

Immunofluorescence Kit

Reactivity

BrdU Incorporated Cells

Applications

Immunohistochemistry (Paraffin & Frozen)

Size

50 Slides

Price (USD)

$376

Background

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.
A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.
Exalpha Biologicals BrdU Immunofluorescence Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and fluorescent staining of these cells which is achieved using a biotinylated anti-BrdU antibody followed by Streptavidin-FL Conjugate

Customer Storage

Store kit at -20°C until first use. Store at 4-8°C thereafter.

Product Specific References


This product has been used in:

1. Sato, S., et al, 'Central control of bone remodeling by neuromedin U' Nature Medicine 2007, 13, , 1234-1240