Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

BrdU (Bromodeoxyuridine), FL549

  • Product Code: X2840K
  • Size: 50 Slides
  • Price (USD): $376

Cat #

X2840K		 Quantity:      

Data Sheet

Product Name

BrdU (Bromodeoxyuridine), FL549

Synonyms

BrdU (Bromodeoxyuridine) Immunofluorescence Kit

Product Type

Immunofluorescence Kit

Reactivity

BrdU Incorporated Cells

Applications

Immunohistochemistry (Paraffin & Frozen)

Size

50 Slides

Price (USD)

$376

Background

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.
A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.
Exalpha Biologicals BrdU Immunofluorescence Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and fluorescent staining of these cells which is achieved using a biotinylated anti-BrdU antibody followed by Streptavidin-FL Conjugate

Customer Storage

Store kit at -20ºC until first use. Store at 4-8ºC thereafter.

Product Specific References


This product has been used in:

1. Sato, S., et al, 'Central control of bone remodeling by neuromedin U' Nature Medicine 2007, 13, , 1234-1240