Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Histone H2A.x (S140)

  • Product Code: X2754P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2754P		 Quantity:      

Data Sheet

Product Name

Histone H2A.x (S140)

Synonyms

H2AFX; H2AX

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot, Immunohistochemistry, EIA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Immunogen

Synthetic peptide derived from human histone H2a.x protein. Sequence is unphosphorylated version of peptide used for Cat. No. X2752P

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of FFPE human bladder carcinoma tissue using Histone H2A.x (S140) antibody (Cat. No. X2754P). Antibody used at 1 µg/ml and visualized using DAB. Pathologists comments: Positive cytoplasmic staining on cancer cells.

Database Links:

SwissProtP16104Human

References

1. Paull, T.T., et al. ?A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage? Curr. Biol., 10, 886-895 (2000)

2. Kobayashi, J., et al. ?NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.? Curr. Biol., 12, 1846-1851 (2002)

3. Stewart, G.S., et al. ?MDC1 is a mediator of the mammalian DNA damage checkpoint.? Nature, 421, 961-966 (2003)

4. Lukas, C., et al. ?Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.? EMBO J., 23, 2674-2683 (2004)