Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Histone H2A.x (S140)

  • Product Code: X2754P
  • Size: 100 µg
  • Price (USD): $355

Cat #

X2754P		 Quantity:      

Data Sheet

Product Name

Histone H2A.x (S140)

Synonyms

H2AFX; H2AX

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot, Immunohistochemistry, EIA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Immunogen

Synthetic peptide derived from human histone H2a.x protein. Sequence is unphosphorylated version of peptide used for Cat. No. X2752P

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of FFPE human bladder carcinoma tissue using Histone H2A.x (S140) antibody (Cat. No. X2754P). Antibody used at 1 µg/ml and visualized using DAB. Pathologists comments: Positive cytoplasmic staining on cancer cells.

Database Links:

SwissProtP16104Human

References

1. Paull, T.T., et al. ‘A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage’ Curr. Biol., 10, 886-895 (2000)

2. Kobayashi, J., et al. ‘NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.’ Curr. Biol., 12, 1846-1851 (2002)

3. Stewart, G.S., et al. ‘MDC1 is a mediator of the mammalian DNA damage checkpoint.’ Nature, 421, 961-966 (2003)

4. Lukas, C., et al. ‘Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.’ EMBO J., 23, 2674-2683 (2004)