Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
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Exalpha Biologicals, Inc.

ATP-Binding Cassette, Sub-Family B (Mdr/Tap), Member 1 (Abcb1)

  • Product Code: X2192P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2192P		 Quantity:      

Data Sheet

Product Name

ATP-Binding Cassette, Sub-Family B (Mdr/Tap), Member 1 (Abcb1)

Synonyms

ABC20, ABCB1, CD243, CLCS, EC 3.6.3.44, GP170, MDR1, P-GP, PGY1

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Purification

Immunoaffinity Purification

Size

50 µg

Price (USD)

$464

Background

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13). Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.

Immunogen

Cytoplasmic domain of human

Positive Control

Human adrenal cortex tissue

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Abcb1 staining of formallin fixed paraffin embedded adrenal cortex tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtP08183Human

References

1. Brant, S.R., et al. 'MDR1 Ala893 polymorphism is associated with inflammatory bowel disease.' Am. J. Hum. Genet. 2003, 73, 1282-1292