Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

CCR10 (G-protein-Coupled Receptor GPR2)

  • Product Code: X2094P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2094P		 Quantity:      

Data Sheet

Product Name

CCR10 (G-protein-Coupled Receptor GPR2)

Synonyms

CC-CKR-10, CCBP2, CCR-10, CCR10, CCR9, CMKBR9, D6, GPR2

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purification

Immunoaffinity Purification

Size

50 µg

Price (USD)

$464

Background

CCR10 belongs to the G-protein coupled receptor 1 family. It is the receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.

Immunogen

A synthetic peptide to the N-terminal domain of human CCR10, linked to KLH

Positive Control

Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

38.4 kDa

Product Image

Image Legend

CCR10 staining of formallin fixed paraffin embedded human skin tissue at a dilution of 5ug/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtP46092Human

References

1. Bonini, J.A., et al 'Cloning, expression, and chromosomal mapping of a novel human CC-chemokine receptor (CCR10) that displays high-affinity binding for MCP-1 and MCP-3.' DNA Cell Biol 1997, 16, 1249-1256

2. Xiong, N., et al. 'CCR10 and its ligands in regulation of epithelial immunity and diseases.' Protein Cell 2012, epub

3. Mirshahpanah, P., et al. 'CCR4 and CCR10 ligands play additive roles in mouse contact hypersensitivity.' Exp. Dermatol. 2008, 17, 30-34