Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Cytochrome P450 1A2

  • Product Code: X2046M
  • Size: 200 µg
  • Availability: In Stock In Stock
  • Price (USD): $232

Cat #

X2046M		 Quantity:      

Data Sheet

Product Name

Cytochrome P450 1A2

Synonyms

CYPIA2, P450-P3, P(3)450, P450 4

Host/Source

Mouse

Clone

3B8C1

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$232

Background

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. NADPH cytochrome 450 reductase is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. NADPH cytochrome P450 reductase is localized to the endoplasmic reticulum where it is also able to transfer electrons to heme oxygenase and cytochrome 5. NADPH cytochrome P450 reductase is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of NADPH cytochrome P450 reductase requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat cytochrome p450 proteins and mouse myeloma Ag8563 cells.

Positive Control

Normal human liver sections

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

58 kDa

Product Image

Image Legend

Left: Western blot using CYP1A2 antibody (Cat. No. X2046M) on recombinant (2) CYP1B1, (3) CYP1A1 and (4) CYP1A2 (0.5 pmol per lane). Right: Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section.

Database Links:

SwissProtP05177Human

References

1. Peng, W.X., et al. (2003). Evidence of the involvement of human liver microsomes CYP1A2 in the mono-hydroxylation of daidzein. Clin. Chim. Acta. 334(1-2); 77-85.

2. Hu, M., et al. Identification of CYP1A2 as the main isoform for the phase I hydroxylated metabolism of genistein and a prodru converting enzyme of methylated isoflavones. Drug Metab Dispos. 31(7); 924-931.

3. Wei, C., et al. CYP1A2 is expressed along with CYP1A1 in the human lung. Cancer Lett. 171(1);113-120.