Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Latest References

Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

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Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

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Exalpha Biologicals, Inc.

Notum

  • Product Code: X1856P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $258

Cat #

X1856P		 Quantity:      

Data Sheet

Product Name

Notum

Synonyms

Pectinacetylesterase homolog, Wingful (Wf)

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$258

Background

Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the / -hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.

Immunogen

Recombinant full-length notum protein from mouse.

Positive Control

HepG2 cell lysate

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

48,602 Da

Product Image

Image Legend

Immunohistochemical staining of normal human cerebellum tissue using Notum antibody (Cat# X1856P) at 20 µg/ml.

Database Links:

SwissProtQ6P988Human
SwissProtQ8R116Mouse

References

1. Kozmik, Z., et al. 'Characterization of mammalian orthologues of the Drosophila osa gene: cDNA cloning, expression, chromosomal localization, and direct physical interaction with Brahma chromatin-remodeling complex.' Genomics, 2001, 73, 140-148

2. Riese, J., et al. 'LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic.' Cell, 1997, 88, 777-787

3. Giraldez, A.J., et al. 'HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.' Dev. Cell, 2002, 2, 667-676