Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Sphingosine Kinase 1, Long Form, Rat Reactive

  • Product Code: X1848P
  • Size: 100 µg
  • Price (USD): $355

Cat #

X1848P		 Quantity:      

Data Sheet

Product Name

Sphingosine Kinase 1, Long Form, Rat Reactive

Host/Source

Rabbit

Isotype

N/A

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.

Immunogen

Synthetic peptide derived from the rat sphingosine kinase 1, long form, protein.

Positive Control

Antibody has been tested on rat kidney lysate.

Formulation

Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -70ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

49843

Product Image

Image Legend

Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X1848P (1 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce’s Super Signal West Femto.

Database Links:

SwissProtQ9NYA1Human
SwissProtQ1HGK5Rat
SwissProtNM_133386Rat

References

1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-636 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73 8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459