Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Sphingosine Kinase 2, Long Form N Terminal HIS Tag

  • Product Code: X1840E
  • Size: 10 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1840E		 Quantity:      

Data Sheet

Product Name

Sphingosine Kinase 2, Long Form N Terminal HIS Tag

Synonyms

Sphk2-L, N-terminal-extended Sphingosine Kinase 2

Host/Source

Sf9 cells

Product Type

Active Enzyme

Reactivity

Human

Applications

Study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

10 µg

Price (USD)

$361

Background

The long form of sphingosine kinase 2 (Sphk2-L) is a species-specific isoform of the Sphk2 enzyme expressed in human but not in mouse. It is an N-terminal extended form of Sphk2, extended by 36 amino acids. It functions as expected, catalyzing the formation of sphingosine 1-phosphate from sphingosine, however it appears to have a decreased ability to inhibit DNA synthesis as compared to Sphk2-S (short form of Sphk2). Under normal cellular conditions, Sphk2-L does not appear to inhibit DNA synthesis, but under serum deprivation, SphK2-L translocates to the nucleus and accumlates. This accumlation may be involved in the cessation of cell proliferation or apoptosis depending on the cell type.

Immunogen

Baclovirus infected Sf9 cells

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

74 kDa

Database Links:

GeneCardNM_020126Human

References

1. Okada, T., et al. 'Involvement of N-terminal-extended form of sphingosine kinase 2 in serum-dependent regulation of cell proliferation and apoptosis.' J. Biol. Chem. 2005, 280, 36318-36325