Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PTPIA2 (693-979)

  • Product Code: X1774E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1774E		 Quantity:      

Data Sheet

Product Name

PTPIA2 (693-979)

Synonyms

Receptor-type tyrosine-protein phosphatase-like N; R-PTP-N; Islet cell antigen 512; ICA 512; Islet cell autoantigen 3; PTPRN

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Size

20 µg

Price (USD)

$361

Background

Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Interacts with phosphorylated SNTB2, protecting it from protein cleavage by CAPN1. Dephosphorylation of SNTB2 upon insulin stimulation disrupts the interaction and results in its cleavage.

Immunogen

E. Coli

Formulation

Provided in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 3 mM DTT, 20 mM glutathione.

Customer Storage

Products should be stored at -80°C. Aliquot to avoid freeze/thaw cycles.

Database Links:

GenBank002846Human

References

1. T. Ort et al., EMBO J. 2001, 20: 4013-23

2. Z. Li et al., J Histochem Chytochem. 2000, 48:761-7