Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

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Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

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Exalpha Biologicals, Inc.

c-Kit-N Terminal His Tag

  • Product Code: X1760E
  • Size: 10 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1760E		 Quantity:      

Data Sheet

Product Name

c- Kit-N Terminal His Tag

Synonyms

CD117, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor

Host/Source

Sf9 cells

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

10 µg

Price (USD)

$361

Background

C-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors . It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells . Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, p85 subunit of PI3 kinase, SHP2 and CrkL, which links c-Kit directly to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders , while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors . Tyrosine 719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

Immunogen

a.a. 544-976 of human c-kit protein, taged with 6 x his

Formulation

Provided in 25 mM Tris-HCl, 100 mM NaCl, pH 8.0, 0.05% Tween-20, 3 mM DTT and 50% glycerol

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

53.4 kDa

Database Links:

SwissProtBC071593Human

References

1. Picardo M Cardinali G. J Invest Dermatol.2011 Jun;131(6):1182-5.

2. Yarden,Y., etal., EMBO J. 6 (11), 3341-3351 (1987)