Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

HDAC6

  • Product Code: X1740E
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1740E		 Quantity:      

Data Sheet

Product Name

HDAC6

Host/Source

Sf9 cells

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Size

50 µg

Price (USD)

$361

Background

Human HDAC6 (GenBank Accession No. BC069243), full length with N-terminal GST tag, MW= 159 kDa, expressed in baculovirus expression system. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

Immunogen

Full length with N-Terminal GST tag

Positive Control

Specific Activity: 50 U/ug. One U =1 pmol/min, Located in the nucleus and Cytoplasm. Not:It is mainly cytoplasmic, where it is associated with microtubules.

Formulation

Formulated in 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 20 mM glutathione, and 10% glycerol.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

159 kDa

Database Links:

GenBankBC069243Human