Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Human Brain Tissue

  • Product Code: X1633C
  • Size: 100 µg
  • Price (USD): $88

Cat #

X1633C		 Quantity:      

Data Sheet

Product Name

Human Brain Tissue

Isotype

N/A

Product Type

Control Lysate

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$88

Background

Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ?g/ml of aprotinin, 5 ?g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ?-mercaptoethanol.

Formulation

Provided in 10% glycerol, 0.063 M Tris-HCl (pH 6.8), 2% SDS and 0.002% bromophenol blue, 5% 2-mercaptoethanol

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles