Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

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Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

DAP12

  • Product Code: X1588P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1588P		 Quantity:      

Data Sheet

Product Name

DAP12

Synonyms

TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein; KARAP; TYROBP

Host/Source

Rabbit

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$244

Background

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (1,2). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (3). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (4,5), as well as presenile dementia and bone cysts in humans (6).

Immunogen

Recombinant DAP12 protein

Positive Control

NKp44 positive cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunoprecipitation using DAP12 antibody on MHC class I (1) and NKp44 (2) positive cells.

Database Links:

SwissProtO54885Mouse
SwissProtO43914Human

References

1. Lanier, L.L. & Bakker, A.B. 'The ITAM-bearing transmembrane adaptor DAP12 in lymphoid and myeloid cell function.' Immunol. Today 2000, 21:611-614

2. Campbell, K.S. & Colonna, M. 'DAP12: a key accessory protein for relaying signals by natural killer cell receptors.' Intl J Biochem Cell Biol 1999, 31:631-636

3. McVicar, D.W., et al. 'DAP12-mediated signal transduction in natural killer cells. A dominant role for the Syk protein-tyrosine kinase.' J Biol Chem 1998, 273:32934-32942

4. Bakker, A.B., et al. 'DAP12-deficient mice fail to develop autoimmunity due to impaired antigen priming.' Immunity 2000, 13:345-353

5. Tomasello E., et al. 'Combined natural killer cell and dendritic cell functional deficiency in KARAP/DAP12 loss of function mutant mice.' Immunity 2000, 13:355-363

6. Paloneva, J., et al. 'Loss-of-function mutations in TYROBP (DAP12) result in presenile dementia with bone cysts.' Nature Genetics 2000, 25:357-361

7. Zou, Wei. et al., 'DAP12 Couples c-Fms Activation to the Osteoclast Cytoskeleton by Recruitment of Syk'. Molecular Cell, Aug 8, 2008, Vol 31 (3) 422-431. WB Using X1588P

8. Campbell K.S. er al. 'NKp44 Triggers NK Cell Activation through DAP12 Association That Is Not Influenced by a Putative Cytoplasmic Inhibitory Sequence'. Journal of Immunology, 2004 Vol 172


Product Specific References


This product has been used in:

1. Zou, W., et al, 'DAP12 Couples c-Fms activation to the osteoclast cytoskeleton by recruitment of Syk' Molecultar Cell 2008, 31, 3, 422-431