Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

MyoD1

  • Product Code: X1414M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1414M		 Quantity:      

Data Sheet

Product Name

MyoD1

Synonyms

Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3

Host/Source

Mouse

Clone

5.2F

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Chicken

Applications

ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Frozen and Paraffn Sections), Immunofluorescense

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$361

Background

Recognizes a protein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing?s sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Positive Control

Rhabdomyosarcoma, SW80 cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Left: Immunohistochemical staining using MyoD1 antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD1 protein with MyoD1 antibody at 2 µg/ml and detected using goat anti-mouse HRP (Cat. No. X1208P) and visualized with Pierce West-Femto substrate.

Database Links:

SwissProtP16075Chicken
SwissProtQ02346Rat
SwissProtP10085Mouse
SwissProtP15172Human

References

1. Thulasi R; et al. Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth and Differentiation, 1996 Apr, 7(4):531-41.

2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9.

3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11.

4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701.

5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9.

6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81.