Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Ku (p70)

  • Product Code: X1390M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $433

Cat #

X1390M		 Quantity:      

Data Sheet

Product Name

Ku (p70)

Host/Source

Mouse

Clone

N3H10

Isotype

IgG2b

Product Type

Monoclonal Antibody

Reactivity

Human, Monkey, Xenopus

Applications

ELISA, Immunofluorescence, Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$433

Background

Recognizes a protein of 70kDa, identified as p70 subunit of Ku. The epitope of this antibody maps between amino acids 506-541 of p70. The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human placental extract designated as PSE1-PL and mouse myeloma NS1 & Sp2/0 cells.

Positive Control

BT474, HeLa, human K562, HL-60, HEp-2, HeLa, Cos-1 cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining using Ku (p70) antibody on formalin fixed, paraffin embedded human tonsil.

References

1. Ajmani AK, et. al. Absence of autoantigen Ku in mature human neutrophils and HL-60 cells and lymphocytes undergoing apoptosis. J. Exp. Med., 1995, 181(6):2049-2058.

2. Wang J, et. al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Molecular Biology Reports, 1993, 18(1):15-28.

3. Knuth, M.W., et al. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem., 1990 Oct 15, 265(29):17911-20.