Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Ku (p70)

  • Product Code: X1390M
  • Size: 100 µg
  • Price (USD): $433

Cat #

X1390M		 Quantity:      

Data Sheet

Product Name

Ku (p70)

Host/Source

Mouse

Clone

N3H10

Isotype

IgG2b

Product Type

Monoclonal Antibody

Reactivity

Human, Monkey, Xenopus

Applications

ELISA, Immunofluorescence, Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$433

Background

Recognizes a protein of 70kDa, identified as p70 subunit of Ku. The epitope of this antibody maps between amino acids 506-541 of p70. The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human placental extract designated as PSE1-PL and mouse myeloma NS1 & Sp2/0 cells.

Positive Control

BT474, HeLa, human K562, HL-60, HEp-2, HeLa, Cos-1 cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining using Ku (p70) antibody on formalin fixed, paraffin embedded human tonsil.

References

1. Ajmani AK, et. al. Absence of autoantigen Ku in mature human neutrophils and HL-60 cells and lymphocytes undergoing apoptosis. J. Exp. Med., 1995, 181(6):2049-2058.

2. Wang J, et. al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Molecular Biology Reports, 1993, 18(1):15-28.

3. Knuth, M.W., et al. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem., 1990 Oct 15, 265(29):17911-20.