Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Pan Keratin (4,5,6,8,10,13,18)

  • Product Code: X1260M
  • Size: 100 µg
  • Price (USD): $244

Cat #

X1260M		 Quantity:      

Data Sheet

Product Name

Pan Keratin (4,5,6,8,10,13,18)

Host/Source

Mouse

Clone

C11

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Reacting with a variety of keratins (4,5,6,8,10,13 and 18) which reacts with a variety of normal reactive and neoplasmic epithelia. Reacting with simple epithelium and both basal and superbasal layers of comfying and non comfying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of human colon cancer tissue using pan Keratin antibody (Cat. No. X1260M) at 2.5 µg/ml.

Database Links:

SwissProtP13647Human

References

1. Folia Biol. 1989, 35, 373-382 2. Neoplasma 1990, 37, 333-342 3. J. Tumor Marker Oncol. 1990, 5, 219 4. Int. J. Cancer 1988, Suppl. 3, 50-55