Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
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Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Bovine Type II Collagen Substrate, FITC Conjugate

  • Product Code: X1099
  • Size: 10 mg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1099		 Quantity:      

Data Sheet

Product Name

Bovine Type II Collagen Substrate, FITC Conjugate

Synonyms

Extracted from bovine articular cartilage

Isotype

N/A

Product Type

Purified Protein

Applications

Substrate

Purification

DEAE Anion Exchange Chromatography

Size

10 mg

Price (USD)

$438

Background

FITC-labeled bovine type II collagen substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied as solution in acetic acid. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).

Immunogen

Bovine articular cartilage

Formulation

Provided as a 1 mg/ml solution in 0.01M acetic acid.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

~1000 kDa

References

1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762

2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307

3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263

4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263

5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334