Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

LFC

  • Product Code: X1089P
  • Size: 250 µg
  • Price (USD): $249

Cat #

X1089P		 Quantity:      

Data Sheet

Product Name

LFC

Synonyms

Rho guanine nucleotide exchange factor 2, ARHGEF2

Host/Source

Sheep

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation, Immunofluorescence

Purification

Ammonium Sulfate Precipitation

Size

250 µg

Price (USD)

$249

Background

Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.

Immunogen

Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

118

Product Image

Image Legend

Western blot analysis using anti-Lfc antibody on HEK-293 cell transfected with vector alone (1), lfc-short (2) and lfc-long (3).

Database Links:

SwissProtQ60875Mouse

References

1. Glaven, J.A., et al. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells. J. Biol. Chem. 1999, 274, 2279-2285 2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381 3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395


Product Specific References


This product has been used in:

1. Chiang, H., et al. 'GEF-H1 controls microtubule-dependent sensing of nuclei acids for antiviral host defenses.' Nature Immunology, Jan 2014, 15(1): 63-71