Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

BrdU (bromodeoxyuridine)

  • Product Code: X1028
  • Size: 100 µg
  • Price (USD): $244

Cat #

X1028		 Quantity:      

Data Sheet

Product Name

BrdU (bromodeoxyuridine)

Synonyms

Anti-Bromodeoxyuridine Mouse MoBU Monoclonal Antibody

Host/Source

Mouse

Clone

MoBU

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

N/A

Applications

Flow Cytometry, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

Immunogen

Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining using BrdU antbody (Cat. No. X1028) on mouse intestine from mice treated with BrdU.

References

1. Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266 3. Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6) 4. Gutschalk, C.M., et al. ‘Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor Promote Malignant Growth of Cells from Head and Neck Squamous Cell Carcinomas In vivo.’ Cancer Res., 66, 8026-8036 (2006).