Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

LEF/TCF/HMG

  • Product Code: T105MS
  • Size: 100 µg
  • Price (USD): $244

Cat #

T105MS		 Quantity:      

Data Sheet

Product Name

LEF/TCF/HMG

Synonyms

TCF domain binding protein, mouse anti human T Cell Factor domain binding protein (includes lef-1)

Host/Source

Mouse

Clone

REMB6

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB6 recognizes the High Mobility Group DNA domain of LEF-1

Immunogen

Full-length recombinant LEF-1 protein

Positive Control

Jurkat cell lysate

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

55-57

Product Image

Image Legend

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.

Database Links:

SwissProtP27782Mouse
SwissProtQ9UJU2Human

References

Hsu SC; Galceran J; Grosschedl R. 'Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.' Mol Cell Biol, 18(8):4807-18 1998 Aug