Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

LEF-1beta catenin

  • Product Code: T100M
  • Size: 250 µg
  • Price (USD): $407

Cat #

T100M		 Quantity:      

Data Sheet

Product Name

LEF-1beta catenin

Synonyms

lef-1, mouse anti human lymphoid enhancer factor-1, T-cell specific transcription factor 1-alpha, TCF-1alpha

Host/Source

Mouse

Clone

REMB1

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence, IHC (paraffin embedded)

Purification

Protein A/G Chromatography

Size

250 µg

Price (USD)

$407

Background

The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the b-catenin binding domain of LEF-1.

Immunogen

Full length recombinant LEF-1 protein.

Positive Control

Jurkat cell lysate

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1.

Database Links:

SwissProtP27782Mouse
SwissProtQ9UJU2Human
SwissProtP27782Mouse
SwissProtQ9UJU2Human

References

Hsu SC; Galceran J; Grosschedl R. 'Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.' Mol Cell Biol, 18(8):4807-18 1998 Aug


Product Specific References


This product has been used in:

1. Driskell. Ryan R., et al, 'Wnt-responsive element controls Lef-1 promotor expressions durig submucosal galnd morphogenesis' American Journal of Physiology Lung Cell Moleculatr Physiology 2004, 287, , 752-763
2. Li. Feng-Qian, et al., 'Lymphoid Enhancer Factor-1 Links Two Hereditary Leukemia Syndromes through Core-binding Factor-alpha Regulation of ELA2' Journal of Biological Chemistry 2004, 279, 4, 2873-2884
3. Kahler, Rachel A., et al., 'Lymphoid Enhancer Factor-1 and Beta Catenin Inhibit Runx2-dependent Transcriptional Activation of the Osteocalcin Promotor' Journal of Biological Chemicstry 2003, 278, 14, 11937-11944
4. Grueneberg, D.A., et al., 'A Functional Screen in Human Cells Identifies UBF2 as an RNA Polymerase II Transcription Factor That Enhances the ß-Catenin Signaling PathwayMolecular Cell Biology' Molecular Cell Biology 2003, 23, , 3936-3950
5. Yokoyama, S., et al, 'Pharmacologic suppression of MITF expression via HDAC inhibitors in the melanocyte lineage' Pigment cell & Melanoma Research 2008, 21, 4, 457-463
6. Holnthoner, W., et al, 'Fibroblast Growth Factor-2 Induces Lef/Tcf-dependent Transcription in Human Endothelial Cell' Journal of Biological chemistry 2002, 277, , 45847-45853
7. Dorfman, David M. et al., 'Loss of Expression of the WNT/Beta Catenin Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell factor-1 (TCF-10 in a Subset of Peripheral T Cel Lymphomas' The American Journal of Pathology 2003, 162, , 1539-1544
8. Shelton, D.N., et al. ‘The Role of LEF1 in Endometrial Gland Formation and Carcinogenesis.’ PLoS One, 7, e40312 (2012)