Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

bax

  • Product Code: A100M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

A100M		 Quantity:      

Data Sheet

Product Name

bax

Synonyms

hbax, mouse anti human bax

Host/Source

Mouse

Clone

2D2

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

The immunogen for the anti-h bax was a synthetic peptide corresponding to amino acids 3 to 16 of the human bax sequence. Bax expression is associated with cell death regulation. The ratio of bax to other bcl-2 family members is believed to regulate apoptosis.

Immunogen

A synthetic peptide corresponding to amino acids 3 to 16 of the human bax sequence

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

21.2 - Bax-alpha 24.2 - Bax-beta

Database Links:

SwissProtQ07814Human (bax-beta)
SwissProtQ07812Human (bax-alpha)

References

1. Y.T. Hsu et. al. (1997) J. Biol. Chem., 272(21): 13829-13834

2. Y. T. Hsu et. al (1997) Proc. Natl. Acad. Sci. USA, 94: 3668-3672

3. Annis, M., et al, 'Bax forms multispanning monomers that oligomerize to permeabilize membranes during apoptosis' EMBO Journal 2005, 24, , 2096-2103

4. Soucie, E.L., et al, 'Myc Potentiates Apoptosis by Stimulating Bax Activity at the Mitochondria' Molecular and Cell Biology 2001, 21, , 4725-4736


Product Specific References


This product has been used in:

1. Annis, M., et al, 'Bax forms multispanning monomers that oligomerize to permeabilize membranes during apoptosis' EMBO Journal 2005, 24, , 2096-2103
2. Soucie, E.L., et al, 'Myc Potentiates Apoptosis by Stimulating Bax Activity at the Mitochondria' Molecular and Cell Biology 2001, 21, , 4725-4736
3. Karch, J., et al. ?Bax and Bak function as the outer membrane component of the mitochondrial permeability pore in regulating necrotic cell death in mice.? eLife, 2, e00772 (2013)