Background
Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type I, cross-reactivity with collagen type II and IV <0.1%, collagen type III and V <1.0%, human fibronectin and laminin <0.1%, mouse laminin <0.1% (RIA at a 1:500 dilution).

Source

Immunogen: Purified collagen type I from human skin

Product
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Secondary Reagents: Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).

Concentration: app. 1 mg/ml

Specificity

Species Reactivity: Human, cross-reactivity with cattle <1.0%, rat and chicken < 0.1% (RIA at a 1:500 dilution)

Applications
IHC(P), IFA, ELISA, RIA, IB/WB

Incubation Time: IHC(P) 60 min at RT or 2-8°C over night

Working Concentration: (purified, lyophilized) IFA 1:80, IHC(P) 1:200 - 1:600, ELISA >1:200 (OD>500)

Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Positive Control: Human skin

Storage
-20°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. (1980) Collagen immunotyping in human liver: Light and electron microscope study. J. Histochem. Cytochem. 28, 1145-1156. 2. Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C. (1982) Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 74, 319-328. 3. Savino W., Itoh T., Imhof B.A., Dardenne M. (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus 8, 245-256. 4. Bedossa P., Bacci J., Lemaigre G., Martin E. (1987) Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 88, 85-89.

Protein Reference(s)

Database Name: UniProt

Accession Number: P02452 (CO1A1_HUMAN); P08123 (CO1A2_HUMAN)

Species Accession: Human