Sheep anti Human chk 1 kinaseCatalog number: K100P
One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
Synonyms: sheep anti human chk 1 kinase, checkpoint kinase 1
Sheep were immunized with a protein corresponding to amino acid sequence 1-474 of human chk-1, having a molecular weight of 54 kDa.
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Western Blot at 1 – 10 ug/ml.
Functional Analysis: Western Blotting
Positive Control: Recombinant proteins conjugated to OVA, GST, His tags and other. Native chk 1 kinase.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Wang, Y., et al. 'CHK1 kinase activity assay.' Methods Mol Biol 2004, 281, 143-151. 2. Yarden, R.I., et al. 'BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage.' Nat Genet 2002, 30, 285-289 3. Nakajo, N., et al. 'Involvement of Chk1 kinase in prophase I arrest of Xenopus oocytes.' Dev Biol 1999, 207, 432-444
Database Name: UniProt
Accession Number: O14757
Safety Datasheet(s) for this product:
Sheep anti Human chk 1 kinase
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|Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.|
Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.