Source
Chickens were immunized with 2 peptides (CGKKTRGGNKASDSGT, CKRPPPKKDREPTTKRPKL), each conjugated to KLH. After multiple immunizations, eggs were collected and IgY was purified from the egg yolk. The antibody was NOT affinity purified.

Product

Product Form: Egg yolk-derived purified IgY

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Purification Method: Egg yolk-derived purified IgY (non affinity purified)

Concentration: See vial for concentration.

Applications
This antibody has been tested in Western blot (see attached image) and a suggested starting dilution is 1:2500, using detector HRP-Donkey anti-IgY (Cat. #DAIgY-HRP) at a 1:3000 dilution. Optimal working dilutions should be determined for your particular assay conditions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Upton, JW, van Dyck, LF and SH Speck. 2005. Characterization of murine gammaherpesvirus 68 v-cyclin interactions with cellular cdks. Virology 341:271-283. 2. Herskowitz, JH, Siegal, AM, Jacoby, MA and SH Speck. 2008. Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection. J. Virol. 82: 3295-3310. 3. Aligo J., Brosnan K, Walker M, Mikkelson ER, Burleson GR, Burleson FG, Volk A, Weinstock D, 2014. Murine gammaherpesvirus-68 (MHV-68) is not horizontally transmitted amongst laboratory mice by cage contact. J. Immunotoxicol. Nov. 21:1-12 4. Santana AL Oldenburg DG, Kirillov V, Malik L, Dong Q, Sinayev R, Marcu KB, White DW, Krug LT. RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency. Pathogens. 2017 Feb 16;6(1). pii: E9. doi: 10.3390/pathogens6010009RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency. Pathogens. 2017 Feb 16;6(1). pii: E9. doi: 10.3390/pathogens6010009 2. Qiwen Dong, Kyle R. Smith, Darby G. Oldenburg, Maxwell Shapiro, William R. Schutt, Laraib Malik, Joshua B. Plummer, Yunxiang Mu, Thomas MacCarthy, Douglas W. White, Kevin M. McBride, Laurie T. Krug Combinatorial Loss of the Enzymatic Activities of Viral Uracil-DNA Glycosylase and Viral dUTPase Impairs Murine Gammaherpesvirus Pathogenesis and Leads to Increased Recombination-Based Deletion in the Viral Genome. American Society for Microbiology, 2018, DOI: 10.1128/mBio.01831-18

Safety Datasheet(s) for this product: