Source
Chickens were immunized with purified Beta-galactosidase from Escherichia coli. After multiple immunizations in Freund's adjuvant, eggs were collected and delipidated. Anti-Beta-galactosidase was immunoaffinity purified using purified Beta-galactosidase immobilized on a solid support.

Product

Product Form: Affinity purified

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.1% Sodium Azide

Concentration: See vial for concentration.

Applications
ELISA: Antibody specificity was verified by ELISA against wells containing 100 ul beta-galactosidase at a concentration of 10 ug/ml. A 1:10,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction. Blotting: Recommended blotting dilutions: 1:1000 – 1: 5000

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Li X, Erclik T, Bertet C, Chen Z, Voutev R, Venkatesh S, Morante J, Celik A, Desplan C. 2013. Temporal patterning of Drosophila medulla neuroblasts controls neural fates. Nature 498:456-462.

Safety Datasheet(s) for this product: