Background
The antibody 5C5 which is directed against sarcomeric alpha-actin labels skeletal and heart muscle cells but not smooth muscle cells. In neoplasms it may be used as a marker of rhabdomyosarcoma. Movement of cell organelles, cells, and organisms depends on the interaction of the cytoskeletal proteins actin and myosin. In birds and mammals up to now six different isoforms of actin have been identified using amino acid sequence analysis and electrophoresis. They are more or less specific for different organs resp. cell types. Four isoforms are known to occur in muscle cells, while two other forms are found in most other cell types. Alpha-Actin sarcomeric (a-sr-1) specific for skeletal- and heart-muscle.

Source

Immunogen: Purified rabbit skeletal muscle actin

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Secondary Reagents: As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Specificity

Species Reactivity: Human, amphibia, bony fish, cattle, guinea pig, reptiles, rabbit, rat, sheep

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: No protease pre-treatment necessary. Blocking of endogenous peroxidase and alkaline phosphatase recommended depending on the detection system in use.

Positive Control: Skeletal muscle

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Skalli O, Gabbiani G, Babai F, Seemayer TA, Pizzolato G, Schurch W. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am. J. Pathol., 130; 515ff (1988) 2. Babai F, Skalli O, Schurch W, Seemayer TA, Gabbiani G. Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms.Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(5):263-77. 3. Hasegawa T, Matsuno Y, Niki T, Hirohashi S, Shimoda T, Takayama J, Watanabe C, Kaneko A, Sano T, Sato M, Suzuki J. Second primary rhabdomyosarcomas in patients with bilateral retinoblastoma: a clinicopathologic and immunohistochemical study. Am J Surg Pathol. 1998 Nov;22(11):1351-60. 4. Eusebi V, Damiani S, Pasquinelli G, Lorenzini P, Reuter VE, Rosai J. Small cell neuroendocrine carcinoma with skeletal muscle differentiation: report of three cases. Am J Surg Pathol. 2000 Feb;24(2):223-30. Review. 5. Tornoczky T, Kalman E, Sapi Z, Orosz Z, Pajor L. Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization: trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor. Virchows Arch. 2001 Feb;438(2):173-80.

Protein Reference(s)

Database Name: UniProt

Accession Number: P68133 (ACTS_HUMAN)

Species Accession: Human

Safety Datasheet(s) for this product: